Multiple kinetic forms of beta-glucuronidase.
نویسندگان
چکیده
منابع مشابه
Application of an improved glucuronidase assay method to the study of human blood beta-glucuronidase.
Previous conditions in which phenolphthalein glucuronide was employed as substrate (1) have proved unsatisfactory for the determination of glucuronidase in plasma, serum, and laked blood cells. Thus, low glucuronidase activities such as frequently occur in plasma could not be accurately measured or even approximated, owing to a combination of circumstances; i.e., too short an incubation period ...
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insulin has been suggested by Cuatrecasas & Illiano (197 1) on the basis of neuraminidase treatment of adipose-tissue cells. Enzymic digestion was reported to have no effect on the total quantity of insulin receptor in the membranes or on the affinity of the receptor for insulin, but abolished the effect of insulin on glucose transport and on lipolysis. As shown in Fig. 1, neuraminidase action ...
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Mouse liver beta-glucuronidase is stabilized within microsomal vesicles by complexation with the accessory protein egasyn. The location of the beta-glucuronidase-egasyn complex and free egasyn within microsomal vesicles was investigated. Surprisingly, it was found that neither the complex nor free egasyn are intrinsic membrane components. Rather, both are either free within the vesicle lumen or...
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A medium containing a chromogenic substrate was developed for the enumeration of Escherichia coli on the basis of beta-glucuronidase activity. In this medium there was an inverse linear relationship between the log initial E. coli concentration and the time taken for the color to reach a threshold optical density of 0.05. This relationship applied even when the E. coli population contained 5% b...
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Gene-mediated enzyme prodrug therapy (GDEPT) seeks to increase the therapeutic index of anti-neoplastic agents by promoting selective activation of relatively nontoxic drug derivatives at sites of specific enzyme expression. Glucuronide prodrugs are attractive for GDEPT due to their low toxicity, bystander effect in the interstitial tumor space and the large range of possible glucuronide drug t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)85964-1